glpX [2019-07-15 08:49:17]

class II fructose-1,6-bisphosphatase, gluconeogenesis

locus: BSU_37090

pI: 5.07

mw: 33.80 kDa

protein length: 321 aa

gene length: 966 bp

function: gluconeogenesis

product: class II fructose-1,6-bisphosphatase

essential: no

ec: 3.1.3.11

synonyms: ywjI

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]

Gene

Coordinates: 3,805,090 3,806,055

The protein

Catalyzed reaction/ biological activity

• D-fructose 1,6-bisphosphate ==> D-fructose 6-phosphate + Pi

Protein family

• FBPase class 2 family (according to Swiss-Prot)

Kinetic information

• K(M) for FBP: 20 M [Pubmed|19270101]

Structure

• [PDB|3D1R] (from ''E. coli'', 49% identity, 68% similarity)[Pubmed|19073594]

Expression and Regulation

Operons

• genes: [gene|523B01B5E7141C7DFC28CE67EB92580F49C20844|murAB]-[gene|0AFCE5C602B073B29DB15DB3CDD153E05E3D02BD|glpX]
  description: [Pubmed|19270101]

  regulation

  • constitutive [Pubmed|19270101]

Biological materials

Mutant

• MGNA-B658 (ywjI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1657 NBRP B. subtilis, Japan]
• BKE37090 ([gene|0AFCE5C602B073B29DB15DB3CDD153E05E3D02BD|glpX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCAACACGATCTCCTC, downstream forward: _UP4_TAACAAACAGAAAAAGCGGG
• BKK37090 ([gene|0AFCE5C602B073B29DB15DB3CDD153E05E3D02BD|glpX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCAACACGATCTCCTC, downstream forward: _UP4_TAACAAACAGAAAAAGCGGG

References

• 19270101,19073594